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Figure 4 | Microbial Cell Factories

Figure 4

From: Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly

Figure 4

ELISA based Nb_An33 quantitation using a 6 × His tag specific detection antibody in order to determine the intra- and extracellular nanobody concentration produced by S. glossinidius harboring pelBNb33 lac and FliCpelBNb33 fliC plasmids at selected time points. Samples were taken every 24 h except during exponential growth (day 3 and 4), 2 samples/24 h were taken (a and b). Values are presented as ng recombinant protein per ml culture medium. The error bars show the ± SD of two biological replicates. Presented data are representative for two independent experiments.

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Microbial Cell Factories

ISSN: 1475-2859

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