Qualitative analysis of intracellular (cytoplasmic and periplasmic) and extracellular Nb_An33 fusion proteins expressed from E. coli (left panel) and S. glossinidius (right panel) harboring the different expression plasmids. E. coli and S. glossinidius samples were not normalized for cell density as these two host cells reach different cell densities at early stationary phase. The localization of the expressed Nb-An33 fusions were analyzed by immunoblotting of cytoplasmic (C) and periplasmic (P) fractions and medium supernatant (S) using an anti-His antibody (1:1000 Serotec) for detection. Presented data are representative for at least three independent experiments. Molecular size markers in kDa are indicated on the gels.