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Figure 1 | Microbial Cell Factories

Figure 1

From: Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly

Figure 1

Schematic presentation of the gene constructs used in this study. Lines represent untranslated DNA regions, filled bars the coding region of Nb_An33 and open bars the coding regions of the respective secretion signals. All gene constructs contain a 6xhis tag (6H) at the carboxy terminal end for purification purposes. Plac and PFliC indicate the lac promoter and the promoter region of the S. glossinidius fliC gene respectively. 5'-UTR indicates the 5' untranslated sequence of fliC including the fliC promotor. FliC1-70 refers to the size in amino acids of the fragment of the S. glossinidius FliC protein containing the predicted signal sequence.

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