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Table 1 Summary Table.

From: Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

reporter enzyme

MUT enzyme

RQ

qs

[mmol·g-1·h-1]

qp

[U·g-1·h-1]

η

[U·mmol-1]

vol. productivity

[U·l-1·h-1]

HRP

benchmark

0.54

- 1.67

1.66

0.99

38.79

 

DAS1

0.56

- 0.51

1.56

3.06

28.35

 

FLD1

0.57

- 1.22

2.01

1.65

49.11

 

TKL1

0.57

- 0.88

1.96

2.22

39.87

CalB

benchmark

0.57

- 1.32

1.39

1.05

30.27

 

DAS1

0.57

- 0.36

0.78

2.15

15.23

 

FLD1

0.57

- 0.70

1.49

2.13

31.27

 

TKL1

0.57

- 0.35

0.12

0.34

2.07

  1. The respiratory quotient (RQ), the specific substrate uptake rate (qs), the specific productivity (qp), the efficiency factor (η) and the volumetric productivity of the benchmark strains (overexpressing either HRP or CalB) and the respective benchmark strains co-overexpressing either of the MUT pathway enzymes DAS1, FLD1 or TKL1 were determined