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Figure 4 | Microbial Cell Factories

Figure 4

From: Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

Figure 4

Transcription analysis of the co-overexpressed MUT pathway genes DAS1 / FLD1 / TKL1 in HRP/CalB overexpressing strains. The transcript levels were normalized to the corresponding transcript levels of ARG4. The increase in AOX2 transcript levels indicated successful induction with methanol. All co-overexpression strains showed elevated transcript levels of the respective target mRNAs compared to the benchmark strains.

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Microbial Cell Factories

ISSN: 1475-2859

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