Table 1 Oligonucleotides used in this study
Primer | Experimental purposes | Primer sequences (5'→3') |
|---|---|---|
EIGF | Overlap with cbh1GR | GGCGGCGGCGGCAGCAAGCGGGCGGGCGGCGGCTATTGG |
EIHF | Overlap with cbh1HR | GAGGCCGCCGCCCGCAAGCGGGCGGGCGGCGGCTATTGG |
EISF | Overlap with signal peptide region of cbh1 | GTTGGCCGTCATCACGGCCTTCTTGGCCACAGCTCGTGCTGCGGGCGGCGGCTATTGG |
EIR | Cloning of gene e1 | GCTCTAGA TCAATGATGATGATGATGATGGCCGACAGGATCGAAAATCG |
pcbh1F | Cloning of cbh1 promoter | GGACTAGT TTTCCCTGATTCAGCGTACCCG |
pcbh1R | Cloning of cbh1 promoter | GCTCTAGA TTGACTATTGGGTTTCTGTGCC |
pcbh1-1R | Site-directed mutagenesis | CGTTGCTTCTGTTTAGCCACAAGCCG |
pcbh1-1F | Site-directed mutagenesis | CGGCTTGTGGCTAAACAGAAGCAACG |
pcbh1-2R | Site-directed mutagenesis | CAACGGCAAAGCCAATCTTCCAATCGTTTGTTTCTTCACTCA |
GFPF | Cloning of egfp | GCTCTAGA GTACCGGTCGCCACCATGGTG |
GFPR | Cloning of egfp | GCTCTAGA TTACTTGTACAGCTCGTCCA |
cbh1F | Cloning of cbh1 | GCTCTAGA ATGTATCGGAAGTTGGCCGTC |
cbh1GR | Overlap with EIGF | ACGGCGGCGGCGGCGACGGCGGCGGCGGCGACGGCGGCGGCGGCGACGGCGGCGGCGGGTCCGTGACTCTCATCATTCC |
cbh1HR | Overlap with EIHF | GGGCGGCGGCCTCGCGGGCGGCGGCCTCGCGGGCGGCGGCCTCGCGGGCGGCGGCCTCCAGGCACTGAGAGTAGTAAGGG |
actF | RT-PCR | TCCTTGCCTTGCGTCATCTAT |
actR | RT-PCR | CACCAATCACTCTCCTGCTACAA |
GFPrtF | RT-PCR | AGTGCTTCAGCCGCTACCC |
GFPrtR | RT-PCR | GATGCCGTTCTTCTGCTTGTC |