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Figure 4 | Microbial Cell Factories

Figure 4

From: Construction of a cellulase hyper-expression system in Trichoderma reesei by promoter and enzyme engineering

Figure 4

SDS-PAGE and western blots of T. reesei RC30-8 and its transformants containing TCE1-FLE or TCE1-RIG. Lane M, protein markers; lanes 1, 2 and 3, the total secreted proteins in the culture filtrate of wild type, transformant with TCE1-FLE and transformant with TCE1-RIG; lane 4 and 5, the purified TCE1-FLE and TCE1-RIG using Novagen Ni-NTA His•Bind® Resin; lane 6 and 7, verification of TCE1-FLE and TCE1-RIG by Western blotting using an anti-His antibody. Unrelated lanes were removed for clarity.

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Microbial Cell Factories

ISSN: 1475-2859

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