Figure 4
The effect of light intensity on H 2 production by co-expression of proteorhodopsin and hydrogenase. Recombinant E. coli BL21(DE3) with two plasmids, pET-HmH and pACYC-RDS, were grown in 500 mL sealed serum bottles containing 350 mL M9 medium. Light intensity was adjusted to 70 μmole photon/(m2·s) or 130 μmole photon/(m2·s). H2 production was measured using GC. Each value and error bars represent the mean of two independent cultures and the standard deviation.