Table 2 Strains and plasmids used in this study

Strains

DH5α

F - Φ80 lacZ ΔM15 recA endA1 Δ (lacZYA-argF) U169 deoR gyrA 96 thi-I hsdR 17 supE 44 relAI

Our laboratory

DH5α/ΔpoxB::tet

ΔpoxB, Tet^R

This work

DH5α/ΔpoxB::1TPHB

ΔpoxB::1tac-phaCAB

This work

DH5α/ΔpoxB::5TPHB

ΔpoxB::5tac-phaCAB

This work

Plasmids

pGreenTIR

Plac- TIR-gfp in pUC1813, Ap^R

[29]

pCL1920

pSC101 replication, Sp^R

[30]

pLC

pLC1920-derived, ploylinker

This work

pLG

pLC-derived, gfp gene from pGreenTIR

This work

p1TG

pLG-derived, 1 single copy of the core-tac- promoter

This work

p2TG

pLG-derived, 2 tandem repeats of the core-tac- promoter

This work

p3TG

pLG-derived, 3 tandem repeats of the core-tac- promoter

This work

p4TG

pLG-derived, 4 tandem repeats of the core-tac- promoter

This work

p5TG

pLG-derived, 5 tandem repeats of the core-tac- promoter

This work

p6TG

pLG-derived, 6 tandem repeats of the core-tac- promoter

This work

p7TG

pLG-derived, 7 tandem repeats of the core-tac- promoter

This work

p8TG

pLG-derived, 8 tandem repeats of the core-tac- promoter

This work

p9TG

pLG-derived, 9 tandem repeats of the core-tac- promoter

This work

p10TG

pLG-derived, 10 tandem repeats of the core-tac- promoter

This work

pCP20

FLP⁺, λ c I857⁺, λ p_R Rep^ts; Amp^R, Cm^R

[31]

pBHR68

pBluescript II SK^--derived, phaCAB genes from Ralstonia eutropha

[32]

pTKIP

Cloning vector, LP regions, I-SceI restriction sites, Amp^R, Km^R

[16]

pTKS/CS

p15A replication, LP regions, I-SceI restriction sites, Cm^R, Tet^R

[16]

pTKRED

pSC101 replication, ParaBAD- driven I-SceI gene, λ-Red, Sp^R,

[16]

pTKIP-1TPHB

pTKIP-derived, 1tac-phbCAB

This work