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Table 3 Bacterial strains and plasmids used in this study

From: Transcriptome profiling of a curdlan-producing Agrobacterium reveals conserved regulatory mechanisms of exopolysaccharide biosynthesis

Strain/Plasmid Description Source
JM109 E. coli K12 strain used for cloning Promega
ATCC 31749 Curdlan-producing Agrobacterium sp. (wild-type) ATCC
ATCC 31749ΔnifR ATCC 31749 mutant with gene knockout of nifR This study
ATCC 31749Δppx1 ATCC 31749 mutant with gene knockout of ppx1 (AGRO_2774) This study
ATCC 31749Δppx2 ATCC 31749 mutant with gene knockout of ppx2 (AGRO_2552) This study
ATCC 31749ΔrpoN ATCC 31749 mutant with gene knockout of rpoN This study
ATCC 31749ΔrrpP ATCC 31749 mutant with gene knockout of rrpP This study
ATCC 31749ΔrelA/spoT ATCC 31749 mutant with gene knockout of AGRO_1497 This study
ATCC 31749Δ0033 ATCC 31749 mutant with gene knockout of AGRO_0033 This study
ATCC 31749Δ0636 ATCC 31749 mutant with gene knockout of AGRO_0636 This study
ATCC 31749Δ1729 ATCC 31749 mutant with gene knockout of AGRO_1729 This study
ATCC 31749Δ3967 ATCC 31749 mutant with gene knockout of AGRO_3967 This study
DH5α/pUCP30T E. coli strain containing suicide vector H. Schweitzer
pGEM-T easy Vector used for cloning internal gene fragments Promega
pUCP30T-nifR Gene knockout plasmid for interruption of nifR This study
pUCP30T-ppx1 Gene knockout plasmid for interruption of ppx1 (AGRO_2774) This study
pUCP30T-ppx2 Gene knockout plasmid for interruption of ppx2 (AGRO_2552) This study
pUCP30T-rpoN Gene knockout plasmid for interruption of rpoN This study
pUCP30T-rrpP Gene knockout plasmid for interruption of rrpP This study
pUCP30T-spoT Gene knockout plasmid for interruption of spoT This study
pUCP30T-0033 Gene knockout plasmid for interruption of AGRO_0033 This study
pUCP30T-0636 Gene knockout plasmid for interruption of AGRO_0636 This study
pUCP30T-1729 Gene knockout plasmid for interruption of AGRO_1729 This study
pUCP30T-3967 Gene knockout plasmid for interruption of AGRO_3967 This study