Plasmids, strains, primers | Description | Sources |
---|---|---|
Plasmids | ||
pMD18-T | 2.7 kb, Ampr | Takara |
pMD18-RCR | 3.7 kb, pMD18-T containing rcr, Ampr | Novagen |
pMD18-SCR | 3.5 kb, pMD18-T containing scr, Ampr | This work |
pMD-PntA | 4.2 kb, pMD18-T containing PntA, Ampr | This work |
pMD-PntB | 4.1 kb, pMD18-T containing PntB, Ampr | This work |
pETDuet™-1 | 5.4 kb, contains two multiple cloning sites, Ampr | Novagen |
pACYCDuet™-1 | 4.0 kb, contains two multiple cloning sites, Cm r | Novagen |
pET-RS | 7.4 kb, pETDuet™-1 containing rcr and scr, Ampr | This work |
pACYC-AB | 6.8 kb, pACYCDuet™-1 containing PntA and PntB, Cm r | This work |
Strains | ||
C. parapsilosis CCTCC M203011 | DNA donors of rcr and scr genes | This laboratory |
E. coli K12 | DNA donors of PntA and PntB genes | This laboratory |
E. coli JM109 | recA 1 supE 44 endA 1 hsdR 17 gyrA 96 relA 1 thi△(Lac-proAB)F’ | This laboratory |
E. coli BL21(DE3) | F-ompT hsdS B (r B -m B -) gal dcm (DE3) | Novagen |
CK | E. coli BL21 bearing pETDuet™-1 and pACYCDuet™-1 | |
RS | E. coli BL21 bearing pET-RS | This work |
AB | E. coli BL21 bearing pACYC-AB | This work |
RSAB | E. coli BL21 bearing pET-RS and pACYC-AB | This work |
Primers | 5′ → 3′ | |
RCR_F | ATCGATCGCATATG TCAATTCCATCAAGCCAGTACGG(NdeI) | This work |
RCR_R | TGACTCTCGAG CTATGGATTAAAAACAACACGACC(Xho I) | This work |
SCR_F | ATCGAATTC GATGGGCGAAATCGAATCTTATTG(Eco RI) | This work |
SCR_R | TGACTGCGGCCGC CTATGGACACGTGTATCCACCGTC(Not I) | This work |
PntA_F | CGCGGATCCATGCGAATTGGCATACCAAG (Bam HI) | This work |
PntA_R | CCCAAGCTT TTAATTTTTGCGGAACATTTTC (Hin dIII) | This work |
PntB_F | CGCGATATC ATGTCTGGAGGATTAGTTAC (Eco RV) | This work |
PntB_R | CCCCTCGAG TTACAGAGCTTTCAGGATTG (Xho I) | This work |