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Table 3 Bioconversions of ( R )-PED to ( S )-isomer with RSAB cells for different NAD + /NADPH ratios

From: Efficient one-step production of (S)-1-phenyl-1,2-ethanediol from (R)-enantiomer plus NAD+–NADPH in-situ regeneration using engineered Escherichia coli

 

No cofactors

Only NAD+

Only NADPH

NAD+ : NADPH

1 : 1

1 : 2

1 : 3

1 : 4

2 : 1

3 : 1

4 : 1

Optical purity (% e.e.)

15.7±0.03

30.2±0.02

24.8±0.04

93.5±0.12

81.2±0.08

76.3±0.10

71.9±0.04

95.4±0.06

97.4±0.11

97.3±0.07

Yield (%)

8.9±0.05

21.8±0.03

16.1±0.02

87.4±0.09

70.3±0.02

67.5±0.02

62.0±0.03

90.1±0.02

95.2±0.02

95.3±0.04

  1. The stereoconversion of (R)-PED to (S)-isomer was performed in the presence of various ratios between NAD+ (1.0 – 2.0 mM) and NADPH (1.0 – 2.0 mM), with both NAD+ and NADPH or with neither, at pH 6.5 and 35°C.
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Microbial Cell Factories

ISSN: 1475-2859

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