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Table 2 Intracellular concentrations of NAD + , NADH, NADP + , and NADPH in recombinant cells during exponential growth

From: Efficient one-step production of (S)-1-phenyl-1,2-ethanediol from (R)-enantiomer plus NAD+–NADPH in-situ regeneration using engineered Escherichia coli

Strains

Intracellular concentrations (μmol/g [dry wt] of biomass) of:

NADH/NAD+ ratio

NADPH/NADP+ ratio

NAD+

NADP+

NADH

NADPH

Total

CK

1.78±0.05

0.13±0.02

0.28±0.01

0.74±0.04

2.93±0.05

0.16±0.02

5.69±0.02

RS

1.71±0.08

0.11±0.01

0.31±0.01

0.68±0.07

2.81±0.08

0.18±0.02

6.18±0.02

AB

2.14±0.04

0.17±0.01

0.34±0.02

0.39±0.06

3.04±0.04

0.16±0.02

2.29±0.02

RSAB

2.19±0.02

0.15±0.01

0.37±0.02

0.42±0.02

3.13±0.12

0.17±0.01

2.80±0.02