Table 2 Effect of metal salts and inhibitors on McoA, McoB and McoG activity

NaN₃ (1 mM)

5.0 ± 0.3

20.0 ± 1.3

16.6 ± 4.2

EDTA (50 mM)

93.8 ± 1.8

82.9 ± 9.8

202.8 ± 33.9

CaCl₂ (50 mM)

63.7 ± 0.4

76.4 ± 4.2

53.3 ± 8.6

MgCl₂ (50 mM)

81.3 ± 0.1

91.0 ± 0.3

78.2 ± 0.5

NaCl (100 mM)

72.5 ± 4.1

83.8 ± 4.2

62.0 ± 9.5

CuSO₄ (0.1 mM)

83.0 ± 10.2

73.1 ± 10.9

14.2 ± 6.5

CuSO₄ (0.5 mM)

51.3 ± 8.1

41.9 ± 1.6

2.9 ± 2.0

CuSO₄ (1 mM)

36.2 ± 6.7

43.0 ± 3.1

n.d.

CuSO₄ (2.5 mM)

31.6 ± 6.4

36.2 ± 3.8

n.d.

SDS (1 mM)

109.0 ± 1.0

139.0 ± 10.1

143.7 ± 27.0

MetOH (50%)

46.1 ± 4.4

29.8 ± 1.3

21.9 ± 5.5

EtOH (50%)

10.8 ± 1.2

34.6 ± 1.1

13.9 ± 4.0

Acetone (50%)

28.8 ± 0.4

46.1 ± 4.2

24.1 ± 1.6