Figure 1
From: Biocatalytic potential of laccase-like multicopper oxidases from Aspergillus niger
SDS-PAGE (a), elution profiles from a Superdex 200 HR 10/30 column (b) of McoA, McoB and McoG. Reference proteins used to calibrate the Superdex 200 HR 10/30 column and calculate the McoA, McoB and McoG molecular mass (c). The gel filtration calibration was performed with the following reference proteins: cytochrome c (12 kDa), myoglobin (18 kDa), α-chymotrypsin (25 kDa), ovalbumin (43 kDa), bovine serum albumin (68 and 136 kDa), 4-hydroxybenzoate 3-hydroxylase (90 kDa), lipoamide dehydrogenase (102 kDa), phenol 2-hydroxylase (152 kDa), catalase (232 kDa), ferritin (440 kDa) and vanillyl-alcohol oxidase (510 kDa). McoA, McoB and McoG were also included in the plot of Kav versus LogMr.