Cloning and recombinant expression of a cellulase from the cellulolytic strain Streptomyces sp. G12 isolated from compost

Table 3 Results of protein identification in the SDS-PAGE in correspondence to the active bands including as significative only proteins identified with at least two peptides

Identified protein (Accession number)	Total score^a	Matched sequence (individual ion score)	Sequence coverage (%)
Endoglucanase I (gi\|544459)	342	LPILVAYNIYNR (44)	19%
		AAAINASIANTPMAR (66)
		YGYTKPFVVDTSR (50)
		QAPNTWVYMDAGNPR (92)
beta-1,4-endoglucanase (gi\|224555766)	418	TEIMIWFNR (25)	41%
		VGPIQPIGSQVGTADVAGR (91)
		WGTSATQCVTATDSGFR (27)
		TDGVNRTEIMIWFNR (43)
		SYPSVFNGCHYTNCSPGTALPAR (33)
		WGTSATQCVTATDSGFRVTQADGSVPTDGAPK (37)
		LGFTLPSGQSVVHAWNASVAPSSGAVTATGPADSPR (49)
		INGISSAPSSISYGYVDNAVYNASYDIWLDPTPR (49)

^a Ions score is -10*Log(P), where P is the probability that the observed match is a random event. Individual ions scores > 48 indicate identity or extensive homology (p<0.05). Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits. (http://www.matrixscience.com/help/interpretation_help.html).

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