From: Cloning and recombinant expression of a cellulase from the cellulolytic strain Streptomyces sp. G12 isolated from compost
Primer
Nucleotide sequence
Annealing temperature (°C)
1 FW
GCCACCGACTCSGGCTTC
62
1 REV
CKGTTGAACCAGATCAT
48-50
2 FW
ATGCCSCGSCTSCGSCACCAC
74
2 REV
CGCCCCCACGGCGAACC
3 FW
CGCCTCGTACGACATCTGG
3 REV
SACAGTSGAGCASGCSGTSCC