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Table 1 Nucleotide sequences of oligonucleotides used to amplify celstrep gene

From: Cloning and recombinant expression of a cellulase from the cellulolytic strain Streptomyces sp. G12 isolated from compost

Primer

Nucleotide sequence

Annealing temperature (°C)

1 FW

GCCACCGACTCSGGCTTC

62

1 REV

CKGTTGAACCAGATCAT

48-50

2 FW

ATGCCSCGSCTSCGSCACCAC

74

2 REV

CGCCCCCACGGCGAACC

62

3 FW

CGCCTCGTACGACATCTGG

62

3 REV

SACAGTSGAGCASGCSGTSCC

74