Table 2 List of oligonucleotide primers used
Primer name | Sequence |
|---|---|
5’TDH3p(HindIII) | 5’-aagcttcagttcgagtttatcattatcaatactgcc-3’ |
3’TDH3p(BamHI) | 5’- ggatccgtgtgtttattcgaaactaagttcttgg-3’ |
5’Sf126 | 5’-atggatattcctgtgattgttacttccgtttcg-3’ |
3’Sf126 XhoI | 5’-ctcgagctagcaatcaagcatgaaggggtc-3’ |
5’GS linker | 5’-gatcctatgtcgacggtagcggcagcggtagcggtagcggcagcgaattctatc-3’ |
3’GS linker | 5’-tcgagatagaattcgctgccgctaccgctaccgctgccgctaccgtcgacatag-3’ |
5’Sf126 | 5’-atggatattcctgtgattgttacttccgtttcg-3’ |
HMG1-BamHI | 5’-ggatccatgccgccgctattcaagggactgaaacagatggcaaa-3’ |
HMG1-TM-R-SalInS | 5’-gtcgacgtcataatttttatatggtaaacgatcagatgctaatacagg-3’ |
5HMG2 EcoRI | 5’-gaattcatgtcacttcccttaaaaacgatagtacat-3’ |
3HMG2-TM-XhoI | 5’-ctcgagataatcataatttctgaagggcaa-3’ |
5’INT1-COD7 | 5’-gtttgccggtggtgcgaacaatagagcgaccatgaccttgaaggtgagacgcagttcgagtttatcattatc-3’ |
3’INT1-COD7 | 5’-ccagtgttgtatgtacctgtctatttatactggtagcaaccctggatctgatatcacctaataact-3’ |
ERG9-pUG F | 5’-agagaaaagacgaagagcagaagcggaaaacgtatacacgtcacatatcacagctgaagcttcgtacgc-3’ |
ERG9-pUG R | 5’-gtacttagttattgttcggagttgtttgtttatgttatttggcgcagactgcataggccactagtggatctg-3’ |
ERG9prom | 5’-ctaaacgagcagcgagaacacgaccac-3’ |
UBC7 pUG F | 5’-gagattatcctaaaaggaacttccctagtaatagtgtaatttggaagggcatagccagctgaagcttcgtacgc-3’ |
UBC7 pUG R | 5’-gtatatagagaacagttaaaaggaagaccaaatgatcattaacctgctacctgctttcagcataggccactagtggatctg-3’ |
UBC7prom | 5’-gaagaacttaccagactgtttcaagt-3’ |
SSM4-R | 5’-tatatgtaaatatgctagcattcattttaaatgtaaggaagaaaacgcctgcataggccactagtggatctg-3’ |
SSM4-F | 5’-tagccaagagtaccactaattgaatcaaagagactagaagtgtgaaagtccagctgaagcttcgtacgc-3’ |
SSM4prom | 5’-gacattgaaaagatgattatgaccgatct-3’ |
PHO86-pUG F | 5’-cgactcttccagttgcacattttttcatagcgaaagtacaagagtaggaacagctgaagcttcgtacgc-3’ |
PHO86-pUG R | 5’-tttattttgttacttcctgcgagttgcaacagaactaacaagatgccatggcataggccactagtggatctg-3’ |