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Table 2 Primers used in this study. Restriction enzyme cut sites are in bold

From: Effects of synthetic cohesin-containing scaffold protein architecture on binding dockerin-enzyme fusions on the surface of Lactococcus lactis

Primer

Sequence (5’ – 3’)

a

TCGAGCGGCCGC GCTGGAACTGGATAAGAC

b

TCGAGATATC TTAGGCTGTACTACGCTATAC

c

TCGAGCGGCCGC GCTTATAGTTGTAGAGGC

d

ATGCGATATC GTCGACTTTATTACATAGGAATCTGGAAG

e

TCGAGCGGCCGC GGATAAAGCCTCGAGCATTG

f

TCGAGATATC TTATCCGGCTGTATTACCTC

g

ATGCGAATTC GGAGACATAGTGAAAGACAATTC

h

ATGCGCGGCCGC TTTACTGTGCGTCGTAATCAC

i

ATGCGCTAGC ATGACCATGATTACGG

J

GCATCAATTG TTTTTGACACCAGACC

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Microbial Cell Factories

ISSN: 1475-2859

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