Figure 4

β-glucuronidase and β-galactosidase activities of L. lactis cells with simultaneous targeting of UidA and LacZ to the scaffold proteins. (A and C) β-glucuronidase and (B and D) β-galactosidase activity resulting from the simultaneous binding of (A and B) UidA-dock1 and LacZ-dock2 with a surface-displayed chimeric scaffold proteins or (C and D) resulting from the simultaneous binding of UidA-dock2 and LacZ-dock1 with a surface-displayed chimeric scaffold proteins. Enzyme activities are reported for a single enzyme bound to the scaffolds (grey bars), when both enzymes are bound (black bars), and with scaffolds lacking cohesin domains (white bars).