Figure 2

In vivo binding of UidA-dock1 and UidA-dock2 on L. lactis cells displaying coh1C3-coh2C2 chimeric scaffold proteins. Cells displaying chimeric scaffolds were tested for their ability to bind UidA-dock1 or UidA-dock2. Scaffolds were comprised of a type 1 cohesin domain, a type 2 cohesin domain, both a type 1 and type 2 cohesin domain, or no cohesin domain. Quantification of enzymes was carried out using the calculated specific activity of purified enzyme, and the known amount of cells in each sample. The number of molecules bound to L. lactis cells corresponds to equivalent amounts of functional cohesin assuming a theoretical 1:1 ratio of dockerin to cohesin binding. Bars represent the number of UidA-dock1 molecules (black bars) and UidA-dock2 molecules (white bars) successfully associated with the scaffolds.