Table 3 Oligonucleotide primers used in this study
From: De novo production of the flavonoid naringenin in engineered Saccharomyces cerevisiae
Name | Sequence (5’-3’) |
|---|---|
Primers for knockouts | Â |
ARO3 KO for | ATGTTCATTAAAAACGATCACGCCGGTGACAGGAAACGCTTGGAAGACTGCAGCTGAAGCTTCGTACG |
ARO3 KO rev | CTATTTTTTCAAGGCCTTTCTTCTGTTTCTAACACCTTCTGCCAATAGCTGCATAGGCCACTAGTGGATCTG |
PDC5 KO for | CATAATCAATCTCAAAGAGAACAACACAATACAATAACAAGAAGAACAAACAGCTGAAGCTTCGTACGC |
PDC5 KO rev | AAAGTAAAAAAATACACAAACGTTGAATCATGAGTTTTATGTTAATTAGCGCATAGGCCACTAGTGGATCTG |
PDC6 KO for | GGCGGCTGTTTGAAGCCATTCTATCTTAATCTTGTGCTATTGCAGTCCTCCAGCTGAAGCTTCGTACGC |
PDC6 KO rev | GTAAGTTTTATTTGCAACAATAATTCGTTTGAGTACACTACTAATGGCGCATAGGCCACTAGTGGATCTG |
ARO10 KO for | GATACTCAAAACAAGTTGACGCGACTTCTGTAAAGTTTATTTACAAGATAACAAAGAAACTCCCTTAAGCCAGCTGAAGCTTCGTACGC |
ARO10 KO rev | GGGTTTTTTATGTGTTAATGAACAGAAAACGAACAATTGGTAGCAGTGTTTTATAATTGCGCCCACAAGTCATAGGCCACTAGTGGATCTG |
ARO4 for | GACGCATTGTTAGCATTG |
ARO4 rev | CGAATTGGCAGTGGTAGAG |
Primers for cloning | Â |
FK7 | GCGACTAGTATGTCTTCATCCAACGCC |
FK8 | GCGGTCGACTCAGTTCTCTTTGGCTAGTT |
FK9 | GCGACTAGTATGATCACTGCAGCTCTAC |
FK10 | GCGGTCGACTCAACAAAGCTTAGCTTTGAG |
FK11 | GAGGCCGGCGCAATTAACCCTCACTAAAG |
FK12 | TGGACTCCAACGTCAAAG |
FK13 | GCGACTAGTATGGTGATGGCTGGTGC |
FK14 | GCGCTCGAGTTAGAGAGGAACGCTGTGC |
FK15 | CGCGCAATTAACCCTCAC |
FK16 | GCGGAGCTCCACTATAGGGCGAATTGGG |
FK29 | GCGTCCCGGAATGCCAGAGTCCAGATTG |
FK30 | GCGTCCCGGAGATATCTTGCCCTTCAGAACCC |
FK52 | GCGACTAGTATGACCTTACAATCCCAAAC |
FK53 | GCGCTCGAGTTAGGCTGGAG |
FK86 | ATCATGAACTTGCGCTCAATTCCGCGCAGAAGGCAATGCTATAAGACCTACGTCCACGGATTGCGCCTAAGACCGGATAAAGCACCGCATAGGGTAATAACTG |
FK87 | ATCGGAAATTCGACCGTGTGCTAGTGCCTATTGATGATCTGGCGGAATGTCTGCCGTGCCATAGCCATGCCTTCACATATAGTGTAATACGGTTATCCACAGAATC |
FK169 | GCGACTAGTATGGTTATGGCTGGTGC |
FK170 | GCGCTCGAGAATTACAATGGAACAGAGTGC |
FK171 | GCGGAGCTCGCTGGAGCTCATAGCTTC |
FK172 | GCGGAGCTCGTACCCAGTATAGCGACC |
Primers for verification | Â |
FK105 | TCTTTCCTGCGTTATCCC |
FK106 | GGCATGTACGGGTTACAG |
FK107 | CGCGTGTACGCATGTAAC |
FK108 | TCCCGTTAGGAACATTGG |
FK109 | GCAAATGCCTGCAAATCG |
FK110 | AACGTGCAGATGGTGATG |
FK111 | CATTATTGAACAGCGTCCAAG |
FK112 | AGAACCGTGGATGATGTG |