Figure 3

Naringenin production in S. cerevisiae. A) Increase in heterologous production of naringenin. B) Phenylethanol production in engineered strains. (○) IMU011 (atPAL1↑, coC4H↑, coCPR1↑, atCHI1↑, atCHS3↑_,at4CL3↑), (●) IMX183 (aro3 Δ ARO4^G226SatPAL1↑, coC4H↑, coCPR1↑, atCHI1↑, atCHS3↑_,at4CL3↑), (□) IMX185 (aro3 Δ ARO4^G226Spdc5 Δ, pdc6 Δ, atPAL1↑, coC4H↑, coCPR1↑, atCHI1↑, atCHS3↑_,at4CL3↑), (■) IMX106 (aro3 Δ ARO4^G226Saro10 Δ, pdc5 Δ, pdc6 Δ, atPAL1↑, coC4H↑, coCPR1↑, atCHI1↑, atCHS3↑_,at4CL3↑), (Δ) non-naringenin producing reference strain CEN.PK2-1C. Cultures were grown in shake flasks on synthetic medium containing 20 g·l^-1 glucose and appropriate growth factors to supplement the auxotrophic requirements of the strains. All cultures were performed in triplicate. Error bars denote standard deviation.