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Table 3 Recombinase-mediated linearization of the chromosome results in cell killing

From: Construction and Characterization of an in-vivo Linear Covalently Closed DNA Vector Production System

R-cell1

Integrated plasmid SS (+/−)2

Cell viability following induction3

   

30oC

42oC

TelN

λ

0.8

0.6

TelN

+

λ

1.0

5.7 X 10-4

TelN

Φ80

0.8

0.4

TelN

+

Φ80

1.0

5 X 10-3

Tel

λ

1.0

1.0

Tel

+

λ

1.0

1.3 X 10-4

Tel

Φ80

1.0

0.8

Tel

+

Φ80

1.0

1.1 X 10-3

  1. 1 R-cells are all W3110 derivatives cured of pAH153 plasmid during construction stage and confirmed for single integration event by PCR.
  2. 2 pAH120 plasmid carrying λ attP grown and prepared under minimal inducing conditions (30°C).
  3. 3 Average of minimum 3 trials. Viability is expressed as a fraction of colonies counted under non-induced (25°C) conditions.