Table 1 Bacteria, Phage and Plasmids

Bacteria

BW23474

F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am), endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510

E. coli Genetic Stock Center (CGSC) # 7838 [39]

DH5α

F-, Δ(argF-lac)169, φ80dlacZ58(M15), ΔphoA8, glnV44(AS), λ^-, deoR481, rfbC1, gyrA96(NalR), recA1, endA1, thi-1, hsdR17

CGSC # 12384

DH5α λpir

F-, Δ(argF-lac)169,endA1, pir⁺, recA1

Gift from Dr. T. Charles; [40]

JM109

F', Δ(gpt-lac)0, glnV44(AS), λ^-, rfbC1, gyrA96(NalR), recA1, endA1, spoT1?, thi-1, hsdR17, pWM5, F128-x

New England Biolabs

W3101

F-, λ^-, galT22, IN(rrnD-rrnE)1, rph-1

CGSC # 4467;

W3110

F-, λ^-, IN(rrnD-rrnE)1, rph-1

CGSC # 4474;[41]

W3110-Cre (W1NN)

F-, λ^-, IN(rrnD-rrnE)1, rph-1 lacZ::cat-cI857-cre (Cm^R)

This study

W3110-TelN (W2NN)

F-, λ^-, IN(rrnD-rrnE)1, rph-1 lacZ::cat-cI857-telN (Cm^R)

This study

W3110-Tel (W3NN)

F-, λ^-, IN(rrnD-rrnE)1, rph-1 lacZ::cat-cI857-tel (Cm^R)

This study

Phages

N15

Wild type (wt) (telN⁺, tos⁺)

Gift from Dr. S. Hertwig; [21]

P1

wt (cre⁺, loxP⁺)

Gift from Dr. B. Funnell; [42]

PY54

wt (tel⁺, pal⁺)

Gift from Dr. S. Hertwig; [21]

Plasmids

pAH120

attP λ integration plasmid (Kn^R)

NBRP [25]

pAH123

cI857-pL-int Φ80 (Ap^R)

NBRP [25]

pAH153

attP Φ80 integration plasmid (Kn^R)

NBRP [25]

pBRINT

lacZ::cat-MCS::lacZ (Cm^R)

NBRP; [37]

pGL2

SV40P-Luc-PolyA-SV40 intron

Promega

pInt(ts)

cI857-pL-int λ (Ap^R)

NBRP [25]

pNN1

cI857-pR-pL-cre-tL (Ap^R)

This study

pNN2

cI857-pR-pL-tel-tL (Ap^R)

This study

pNN3

cI857-pR-pL-telN-tL (Ap^R)

This study

pNN4

lacZ::cat- cI857-pR-pL-cre-tL::lacZ (Cm^R)

This study

pNN5

lacZ::cat- cI857-pR-pL-tel-tL::lacZ (Cm^R)

This study

pNN6

lacZ::cat- cI857-pR-pL-telN-tL::lacZ (Cm^R)

This study

pNN7

pGL2-egfp switched for luc

This study

pNN8

pNN7 + SS (upstream of SV40 promoter)

This study

pNN9

pNN8-SS (2SS) (second SS downstream of SV40 polyA sequence)

This study

pNN10

pAH120 (SS⁺)

This study

pNN11

pAH153 (SS⁺)

This study

pPL451

cI857-pR-pL-MCS-tL (Ap^R)

Accession # AB248919 National Bioresource Project (NBRP); [43]