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Figure 5 | Microbial Cell Factories

Figure 5

From: Construction and Characterization of an in-vivo Linear Covalently Closed DNA Vector Production System

Figure 5

Effect of Tel-mediated chromosomal disruption on cell morphology. Tel-mediated disruption of the host chromosome results in a contracted cellular morphology. Tel+ R-Cells possessing an integrated plasmid that was SS+ (pal+) or SS— (pal—) were grown in LB media at 30°C (no Tel production) to early log phase A600 = 0.2 , then were divided in two tubes and grown separately at 30°C or 42°C to reach to late log phase A600 = 0.8. Bacterial smears were then prepared on the slide and heat fixed and gram stained. Pictures of bacteria were taken at 1000X magnification. A. SS— integrants at 30°C where tel expression is repressed; B. SS— integrants at 42°C where tel is induced but the pal target site is absent; C. SS+ integrants at 30°C where pal target site is present but tel expression is repressed; D. SS+ integrants at 30°C where tel is induced and present to act on the chromosomally integrated pal target site.

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