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Figure 4 | Microbial Cell Factories

Figure 4

From: Construction and Characterization of an in-vivo Linear Covalently Closed DNA Vector Production System

Figure 4

Schematic representations of linear and circular vector integration events. A mini vector that undergoes a single recombination event with the host chromosome is rare due to the removal of all elements except the cistron containing the GOI expression cassette and the flanking Super Sequence sites. A. Lcc plasmid DNA vector integration event would result in chromosomal ”break” at the site of integration, whereby the chromosome cannot be replicated or segregated and the integrated mammalian cell cannot divide due to separation of centromere from telomere. B. A “minicircle” vector can integrate into a non-essential region of the host chromosome without “breaking” the chromosome, whereby the cell is able to continue dividing with the insertion.

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