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Figure 3 | Microbial Cell Factories

Figure 3

From: Construction and Characterization of an in-vivo Linear Covalently Closed DNA Vector Production System

Figure 3

Conditional processing of the parent plasmid DNA vectors. A. R-cell conditional processing of the parent pNN9 plasmid into mini vectors. Under induced conditions, R-cells lead to the production of mini lcc (TelN- or Tel-cell) and mini ccc (Cre-cell) DNA vectors by recombinase activity on its target site encoded within the 2 Super Sequence sites on pNN9. Processing of the parent plasmid DNA results in production of two species—the mini DNA vector and the mini plasmid backbone. B. Processing of the parent plasmid construct into mini lcc and mini ccc vectors. Efficiency of processing of the pNN9 plasmid into mini lcc DNAs (Tel) and “minicircles” (Cre) after plasmid extraction from R-cells under induced (42°C) conditions. Schematics adjacent to each bands show the DNA constructs and expected conformation. C. In vivo Tel-pal recombination efficiency versus TelN-telRL. Efficiency of processing of the pNN9 plasmid into mini lcc DNA vectors in Tel+ versus TelN+ R-cells.

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