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Table 3 Plasmids and strains used in this study

From: Artificial biosynthesis of phenylpropanoic acids in a tyrosine overproducing Escherichia coli strain

Plasmid or strain

Relevant Characteristics

Source

Plasmids

pET-28a(+)

f1 ori, T7 promoter, KanR

Novagen

pET-22b(+)

f1 ori, T7 promoter AmpR

Novagen

pET-TAL

pET-28a(+) carrying TAL from Saccharothrix espanaensis

Choi.et al.[10]

pET-opTAL

pET-28a(+) carrying codon-optimized S. espanaensis TAL

This study

pET-Sam5

pET-28a(+) carrying Sam5 from S. espanaensis

Choi.et al.[10]

pET-COM

pET-28a(+) carrying COM from Arabidopsis thaliana

Choi.et al.[10]

pET-T5

pET-28a(+) carrying S. espanaensis TAL and Sam5

This study

pET-opT5

pET-28a(+) carrying codon-optimized TAL and S. espanaensis Sam 5

This study

pET-T5M

pET-28a(+) carrying S. espanaensis TAL, S. espanaensis Sam5, and A. thaliana COM

Choi.et al.[10]

pET-opT5M

pET-28a(+) carrying codon-optimized TAL, S. espanaensis Sam5, and A. thaliana COM

This study

pACYCDuet-1

p15A ori, double T7 promoter, CmR

Novagen

pET28-tyrA*

pET-28a(+) carrying codon-optimized tyr Afbr

This study

pET22-aroG*

pET-22b(+) carrying codon-optimized aro Gfbr

This study

pAD-AG

pACYDuet-1 carrying codon-optimized tyr Afbr and aro Gfbr

This study

Strains

E. coli DH5a

cloning host

Invitrogen

E. coli C41(DE3)

derivative strain of E. coli BL21(DE3)

Miroux B & Walker JE[23]

ΔtyrR

tryR gene in-frame deletion mutant of E. coli C41(DE3)

This study

pAD-AG/ΔtyrR

E. coli C41(DE3) ΔtryR :: pAD-AG; tyrosine overproducing strain

This study