From: Artificial biosynthesis of phenylpropanoic acids in a tyrosine overproducing Escherichia coli strain
Plasmid or strain | Relevant Characteristics | Source |
---|---|---|
Plasmids | ||
pET-28a(+) | f1 ori, T7 promoter, KanR | Novagen |
pET-22b(+) | f1 ori, T7 promoter AmpR | Novagen |
pET-TAL | pET-28a(+) carrying TAL from Saccharothrix espanaensis | Choi.et al.[10] |
pET-opTAL | pET-28a(+) carrying codon-optimized S. espanaensis TAL | This study |
pET-Sam5 | pET-28a(+) carrying Sam5 from S. espanaensis | Choi.et al.[10] |
pET-COM | pET-28a(+) carrying COM from Arabidopsis thaliana | Choi.et al.[10] |
pET-T5 | pET-28a(+) carrying S. espanaensis TAL and Sam5 | This study |
pET-opT5 | pET-28a(+) carrying codon-optimized TAL and S. espanaensis Sam 5 | This study |
pET-T5M | pET-28a(+) carrying S. espanaensis TAL, S. espanaensis Sam5, and A. thaliana COM | Choi.et al.[10] |
pET-opT5M | pET-28a(+) carrying codon-optimized TAL, S. espanaensis Sam5, and A. thaliana COM | This study |
pACYCDuet-1 | p15A ori, double T7 promoter, CmR | Novagen |
pET28-tyrA* | pET-28a(+) carrying codon-optimized tyr Afbr | This study |
pET22-aroG* | pET-22b(+) carrying codon-optimized aro Gfbr | This study |
pAD-AG | pACYDuet-1 carrying codon-optimized tyr Afbr and aro Gfbr | This study |
Strains | ||
E. coli DH5a | cloning host | Invitrogen |
E. coli C41(DE3) | derivative strain of E. coli BL21(DE3) | Miroux B & Walker JE[23] |
ΔtyrR | tryR gene in-frame deletion mutant of E. coli C41(DE3) | This study |
pAD-AG/ΔtyrR | E. coli C41(DE3) ΔtryR :: pAD-AG; tyrosine overproducing strain | This study |