Figure 3
From: Artificial biosynthesis of phenylpropanoic acids in a tyrosine overproducing Escherichia coli strain
Comparison of TAL activity. Concentrations of 4-coumaric acid in strains with pET-TAL or pET-opTAL cultures in an M9 medium containing glucose 15 g/L (Black bar and white bar) or 40 g/L (bo1d diagonal bar and thin diagonal bar). Each cultivation was done at least in triplicate, and the standard deviations are shown. Error bars indicate standard errors of the means. The codon-optimized TAL activity and glucose effect were compared by single-factor ANOVA (P < 0.05) using Microsoft Excel. Different stars codes (*, **) indicate significant differences (*, P = 9.44E-5; **, P = 1.62E-4).