Figure 4From: Rationally re-designed mutation of NAD-independent l-lactate dehydrogenase: high optical resolution of racemic mandelic acid by the engineered Escherichia coliOptimization of pH and temperature for biocatalysis. (A) Optimization of pH. (B) Optimization of temperature. Unshaded bars represent the production of benzoylformic acid in 4 h. Shaded bars represent the production of benzoylformic acid in 10 h. Values are the mean ± SD of 3 separate determinations.Back to article page