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Table 1 The pTGR series of plasmids used in this study

From: Design and testing of a synthetic biology framework for genetic engineering of Corynebacterium glutamicum

Plasmid

Promoter

RBS

Gene

Replicon

pTGR1

-

-

eGFP

pGA1 mini replicon

pTGR2

-

sod

eGFP

pGA1 mini replicon

pTGR3

sod

sod

eGFP

pGA1 mini replicon

pTGR4

csp B

sod

eGFP

pGA1 mini replicon

pTGR5

tac

sod

eGFP

pGA1 mini replicon

pTGR6

tac

lacZ

eGFP

pGA1 mini replicon

pTGR7

tac

csp B

eGFP

pGA1 mini replicon

pTGR8

tac

sod / sod

mCherry /eGFP

pGA1 mini replicon

pTGR9

tac / sod

sod / sod

eGFP/ mCherry

pGA1 mini replicon

pTGR10

tac

lacZ / sod

mCherry /eGFP

pGA1 mini replicon

pTGR11

tac

lacZ / lacZ

mCherry /eGFP

pGA1 mini replicon

pTGR12

tac

sod / lacZ

mCherry /eGFP

pGA1 mini replicon

pTGR13

sod / tac

sod / sod

eGFP/ mCherry

pGA1 mini replicon

pTGR14

tac / cspB

sod / sod

eGFP/ mCherry

pGA1 mini replicon

pTGR15

cspB / tac

sod / sod

eGFP/ mCherry

pGA1 mini replicon

pTGR16

tac

sod

eGFP

pNG2 minimal replicon

pTGR17

tac

sod

eGFP

pCRY4 minimal replicon

  1. In all the cases the ColE1 origin of replication from E. coli, and the rrnBT1 and rrBT2 in tandem transcriptional terminators, the Tn5 kanamycin resistance marker and a cassette for the expression of the LacI repressor were used.