Figure 5

Effect of IPTG concentration and plasmid copy number on eGFP expression in C. glutamicum using the pTGR system. (A) Fluorescence intensity of eGFP relative to OD during the growth of C. glutamicum using different IPTG concentration to drive the expression of the reporter gene. (B) Fluorescence intensity of eGFP relative to OD testing three different replicons. pGA1 (medium copy number), pCRY4 (low copy number) and pNG2 (low copy number.) Cultures were grown in BHIS medium and supplemented with the indicated concentration of IPTG at OD₅₉₀ = 0.5. Values shown are means of three independent determinations. The standard deviations were in all the cases less than 10% of the corresponding means.