Figure 4

Comparison of the co-expression levels of eGFP and mCherry genes in C. glutamicum. (A) the genes were assembled in operons with the indicated RBSs controlling the expression. In all cases operons are transcribed from the tac promoter. (B) the genes were co-expressed from two transcriptional units and the indicated promoter drives the expression of each gene. Fluorescence intensity of eGFP and mCherry relative to OD after 24 h of incubation of cultures is shown. Cultures were grown in BHIS medium and supplemented with 0.5 mM IPTG when the tac promoter was used. Values shown are means of three independent determinations. The standard deviations were in all the cases less than 10% of the corresponding means.