Figure 2From: Towards scalable production of a collagen-like protein from Streptococcus pyogenes for biomedical applicationsSDS-PAGE analysis of protein expression. (A) Protein expression in shake flask cultures. MW, molecular weight standards; DM, defined medium; YT, complex medium (2xYT) and purified VCL protein standards 0.7 to 2.0 μg. (B) Protein expression time course, fed-batch process with stepwise temperature reduction after induction. MW, molecular weight standards; I, pre-induction; I + 1 to I + 24 hours after induction; VCL, purified VCL standard.Back to article page