Skip to main content
Figure 3 | Microbial Cell Factories

Figure 3

From: Modulation of endogenous pathways enhances bioethanol yield and productivity in Escherichia coli

Figure 3

Improvement in cell growth upon modulation of expression of ack gene in the engineered SSY09 strain. SSY09 strain having plasmid pZSack was grown in Hungate tube completely filled with defined media + 2.5g/l glucose (A) or 2.5g/l xylose (B) at 37°C for 24 hr. Acetate kinase expression was induced with 0, 0.1, 100 ng/ml of anhydrotetracycline. E. coli B and SSY09 bearing pZS*mcs plasmid were used as positive and negative control, respectively. Results indicate improvement in growth of PZSack transformed cells as compared to control plasmid and less acetate production as compared to wild type strain. Strain description: SSY09 - PgapAPDH ΔldhA ΔfrdA Δack ΔpflB.

Back to article page