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Figure 2 | Microbial Cell Factories

Figure 2

From: Modulation of endogenous pathways enhances bioethanol yield and productivity in Escherichia coli

Figure 2

Functional characterization of promoter engineered E. coli B strains (SSY01-05, see Table1for genotype). Effect of PDH operon promoter replacement on (A) pyruvate dehydrogenase activity and (B) ethanol production was monitored. Cells were grown anaerobically in completely filled Hungate tubes and were harvested and permeabilized to measure PDH activity. The supernatant of the culture was used to analyze metabolite concentration via HPLC. Strain description for changed PDH promoter: SSY01 – PldhAPDH, SSY02 –PfrdAPDH, SSY03 – PpflBPDH, SSY04 – PadhEPDH, SSY05 – PgapAPDH.

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