Table 4 Vectors and plasmids used in this study
From: The LIKE system, a novel protein expression toolbox for Bacillus subtilis based on the liaI promoter
Plasmid | Genotype/propertiesa | Primer pair(s) used for cloning | Reference |
|---|---|---|---|
pDG1662 | cat, spc, bla, amyE' … 'amyE integrative vector |  | [28] |
pGP380 | erm, bla, Strep-Tag, PdegQ36, replicative vector | Â | [29] |
pMAD | erm, ori(pE194-Ts), MCS-P clpB -bgaB, ori(pBR322), bla | Â | [43] |
pSG1151 | bla, cat, gfpmut1 | Â | [44] |
pAT6200 | pDG1662 derivative; spc gene deleted | Â | This work |
pLIKE-int | pAT6200 derivative; PliaI(opt); integrative protein expression vector | TM2064/TM1980 | This work |
pLIKE-rep | pGP380 derivative; PliaI(opt); replicative protein expression vector | TM1991/TM1992 | This work |
pAT6203 | pLIKE-int, PliaI(opt) translationally fused to gfp | TM1981/TM1982 | This work |
pAT3803 | pLIKE-rep, PliaI(opt) translationally fused to gfp | TM1993/TM1994 | This work |
pAT101 | pMAD ΔliaIH up/down overlap | TM2130/ TM2131, TM1055/ TM1058 | This work |
pAT102 | pMAD ΔliaIHTerminator up/down overlap | TM2130/ TM2132, TM2133/ TM2134 | This work |
pKSLIKEr01 | pLIKE-rep, PliaI(opt) translationally fused to His6-ydfG | TM2545/TM2536 | This work |
pKSLIKEi01 | pLIKE-int, PliaI(opt) translationally fused to His6-ydfG | TM2535/TM2536 | This work |