Table 2 Bacterial strains used in this study
From: The LIKE system, a novel protein expression toolbox for Bacillus subtilis based on the liaI promoter
Strain | Relevant genotype | Source and/or reference |
|---|---|---|
E. coli DH5α | recA 1 endA 1 gyrA 96 thi hsdR 17(rK- mK+) relA 1 supE 44 ϕ80ΔlacZ ΔM15 Δ(lacZYA-argF)U169 | Laboratory stock |
Bacillus subtilis | Â | Â |
W168 | Wild type, trpC 2 | Laboratory stock |
HB0933 | W168 att SPβ2∆2 trpC 2, liaR::kan | [18] |
TMB016 | W168 amy E::(cat P liaI -lacZ) | [21] |
TMB020 | HB0933 amyE::(cat P liaI -lacZ) | [21] |
TMB329 | W168 ∆liaF (clean deletion) | [23] |
TMB331 | TMB329 amyE::(cat P liaI -lacZ) | This work |
TMB408 | W168 amyE::pSJ5101 (P liaI -gfp) | S. Jordan |
TMB604 | W168 ΔP liaI -liaIH (clean deletion) | [23] |
Bsu-LIKE1 (TMB1151) | W168 ∆liaIH (clean deletion) | This work |
Bsu-LIKE2 (TMB1152) | W168 ∆liaIH-terminator (clean deletion) | This work |
TMB1172 | W168 amyE::pAT6203 (pLIKE-int PliaI(opt)-gfp) | This work |
TMB1176 | W168 pAT3803 (pLIKE-rep PliaI(opt)-gfp) | This work |
TMB1174 | TMB604 amyE::pAT6203 (pLIKE-int PliaI(opt)-gfp) | This work |
TMB1178 | TMB604 pAT3803 (pLIKE-rep PliaI(opt)-gfp) | This work |
TMB1153 | TMB1151 amyE::pAT6203 (pLIKE-int PliaI(opt)-gfp) | This work |
TMB1342 | TMB1151 pAT3803 (pLIKE-rep PliaI(opt)-gfp) | This work |
TMB1318 | TMB1152 amyE::pAT6203 (pLIKE-int PliaI(opt)-gfp) | This work |
TMB1343 | TMB1152 pAT3803 (pLIKE-rep PliaI(opt)-gfp) | This work |
TMB1566 | TMB1151 pKSLIKEr01 (pLIKE-rep PliaI(opt)-His6-ydfG) | This work |
TMB1570 | TMB1152 pKSLIKEi01 (pLIKE-int PliaI(opt)-His6-ydfG) | This work |