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Table 1 Effect of mutations in the liaIH operon on the expression of translational P liaI (opt) - gfp fusions

From: The LIKE system, a novel protein expression toolbox for Bacillus subtilis based on the liaI promoter

Strain

Relevant genotypea

Promoter activity (fluorescence)b

Expression plasmid

Strain background

TMB408

amyE:: pSJ5101 (P liaI -gfp)

(WT168) P liaI liaIHTerm+

264

TMB1172

amyE:: pLIKE-int+gfp

(WT168) P liaI liaIHTerm+

1440

TMB1174

(TMB604) ∆P liaI liaIH

958

TMB1153

(TMB1151) ∆liaIH

1080

TMB1318

(TMB1152) ∆liaIHTerm

1416

TMB1176

pLIKE-rep+gfp

(WT168) P liaI liaIHTerm+

9570

TMB1178

(TMB604) ∆P liaI liaIH

3372

TMB1342

(TMB1151) ∆liaIH

10607

TMB1343

(TMB1152) ∆liaIHTerm

10870

  1. a The terminator downstream of liaH is abbreviated “Term”, its presence is indicated by a “+”. b Promoter activities were calculated taking the derivative of the fluorescence divided by the OD600 (dGFP/dt/OD600) at each time point.