Figure 5From: The LIKE system, a novel protein expression toolbox for Bacillus subtilis based on the liaI promoterOverproduction of YdfG using the LIKE system. 14% tricine SDS-PAGE of total proteins (10 μg/lane) of strains TMB1566 (YdfG-rep), TMB1570 (YdfG-int), and TMB1151 (control) treated with and without 30 μg ml-1 bacitracin for 30 min. The position of the band corresponding to the protein YdfG is marked. M, molecular weight marker (in kDa).Back to article page