Figure 4From: The LIKE system, a novel protein expression toolbox for Bacillus subtilis based on the liaI promoterConcentration-dependent induction of the P liaI (opt) in B. subtilis W168 cultures. (A) Growth, absolute fluorescence and (B) promoter activity of strains carrying translational fusion of Plia I(opt)-gfpmut1 on plasmids pLIKE-int and pLIKE-rep treated with different concentration of bacitracin. Growth profiles are shown without symbols and expression by symbols: (◊) 1 μg mL-1, (□) 3 μg mL-1, (■) 10 μg mL-1, (○) 30 μg mL-1, (●) 50 μg mL-1, (∆) 100 μg mL-1. Vertical dotted line indicates the point of mid-log phase (OD600~0.4-0.5) when bacitracin was added. Fluorescence is expressed in arbitrary units (AU) (C) Western blot analysis revealed the amount of GFP produced by cells harboring pAT6203 (pLIKE-int) and pAT3803 (pLIKE-rep) 90 min post induction.Back to article page