Figure 3
From: The LIKE system, a novel protein expression toolbox for Bacillus subtilis based on the liaI promoter
Growth, absolute fluorescence (A) and promoter activity (B) of strains carrying translational fusion of P liaI - gfpmut1 and P liaI (opt)- gfpmut1 . Growth profiles are shown without symbols and expression by symbols: (○) (W168), (□) (TMB604), (◊) (TMB1151), (∆) (TMB1152) and (●) (TMB408). Vertical dotted line indicates time point of bacitracin addition (final conc. 30 μg mL-1; OD600~0.4-0.5). Fluorescence is expressed in arbitrary units (AU) (C) Western blot analysis of the cytoplasmic fractions of cells expressing the same fusions probed with LiaH or GFP antisera. Lanes 1–2, protein expression with native modified P liaI ; 3–10, with optimized PliaI(opt) in the absence (−) and presence (+) of bacitracin (final conc. 30 μg mL-1).