Figure 1
From: The LIKE system, a novel protein expression toolbox for Bacillus subtilis based on the liaI promoter
Activity of the native liaI promoter (P liaI ) as monitored by (A) promoter-reporter gene fusions and (B) SDS-PAGE. (A) β-Galactosidase reporter assays of a P liaI -lacZ fusion in the wild type W168 (TMB016) and the corresponding liaF and liaR mutants (TMB331 and TMB020, respectively) in the presence and absence of bacitracin (Bac; final concentration 50 μg/ml). The assay was performed as previously described [21], the promoter activity is expressed as Miller units (β-galactosidase activity normalized against cell density). (B) SDS-PAGE of the soluble protein fraction (15 μg/lane) of the wild type (WT) and isogenic liaF and liaR mutants, challenge for 30 min with bacitracin as described above. The position of the band corresponding to the LiaH protein is marked. The identity of LiaH was verified by mass spectroscopy. M, molecular weight markers.