Table 3 Reported purification procedures of nitrilase

Pseudomonas sp.

DEAE-cellulose; Ammonium sulfate fractionation; Starch electrophoresis

400

12

14

[28]

Nocardia sp. NCIB 11215

DEAE-cellulose; Ammonium sulfate fractionation; Sephadex G-200

15.82

1.74

9.1

[123]

Pseudomonas sp. 13

Ethanol fractionation; Ammonium sulfate fractionation; DEAE-cellulose; 1^st crystallization; 2^nd crystallization

656

3280

21

[31]

F. oxysporum f sp. melonis

DEAE-Sephacel; TSK-phenyl 5 PW

39.72

143

50

[64]

R. rhodochrous K22

DEAE-Sephacel; Ammonium sulfate fractionation; Phenyl-Sepharose CL-4B; Cellulofine GCL-2000 superfine

8.3

0.737

9.08

[40]

Acinetobacter sp. AK 226

1^st DEAE-cellulose; 1^st Hydroxyapatite; 2^nd DEAE-cellulose; 2^nd Hydroxyapatite; Sephacryl S-400

4.41

0.156

20.1

[37]

A. faecalis ATCC 8750

Ammonium sulfate fractionation; Phenyl-Sepharose CL-4B; DEAE-cellulose

29.0

3.10

17.9

[38]

C. testosteroni sp.

Q-Sepharose; Superdex 200; Hydroxyapatite

60

68

79

[35]

R. rhodochrous NCIMB 11216

Ammonium sulfate precipitation; Anion-exchange FPLC; Sepharose Q; Suparose 12 HR 10/30

62

10.6

9.5

[43]

R. rhodochrous PA-34

Ammonium sulfate fractionation; 1^st Sephacryl S-300 HR; 2^nd Sephacryl S-300 HR; DEAE Toyopearl 650S

14.10

3.52

34.8

[39]

P. fluorescens DSM 7155

Phenyl-Sepharose FF; Mono Q; Superose 12

259

90

10

[44]

A. facilis 72W

Ammonium sulfate precipitation; Sephadex G-25; Q-Sepharose

10.5

21

65

[48]

P. putida MTCC 5110

Ammonium sulfate fractionation; Superdex 200; Q-sepharose; Phenyl sepharose

35.01

3.26

10.15

[46]

A. niger K10

Ammonium sulfate precipitation; Sephacryl S-200; Q-Sepharose

18.7

91.6

24.3

[66]

P. abyssi GE5

Heat treatment; Q-Sepharose

23.85

141.9

88

[47]

F. solani O1

Phenyl sepharose; Sephacryl S-200; Q-Sepharose

9.9

156

25.9

[67]

F. solani IMI 196840

Phenyl sepharose; Sephacryl S-200; Q-Sepharose

20.3

144.0

26.9

[69]