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Table 3 Reported purification procedures of nitrilase

From: Nitrilases in nitrile biocatalysis: recent progress and forthcoming research

Organisms Purification processa(In order) Purification fold Specific activity (U·mg-1) Yield (%) Reference
Pseudomonas sp. DEAE-cellulose; Ammonium sulfate fractionation; Starch electrophoresis 400 12 14 [28]
Nocardia sp. NCIB 11215 DEAE-cellulose; Ammonium sulfate fractionation; Sephadex G-200 15.82 1.74 9.1 [123]
Pseudomonas sp. 13 Ethanol fractionation; Ammonium sulfate fractionation; DEAE-cellulose; 1st crystallization; 2nd crystallization 656 3280 21 [31]
F. oxysporum f sp. melonis DEAE-Sephacel; TSK-phenyl 5 PW 39.72 143 50 [64]
R. rhodochrous K22 DEAE-Sephacel; Ammonium sulfate fractionation; Phenyl-Sepharose CL-4B; Cellulofine GCL-2000 superfine 8.3 0.737 9.08 [40]
Acinetobacter sp. AK 226 1st DEAE-cellulose; 1st Hydroxyapatite; 2nd DEAE-cellulose; 2nd Hydroxyapatite; Sephacryl S-400 4.41 0.156 20.1 [37]
A. faecalis ATCC 8750 Ammonium sulfate fractionation; Phenyl-Sepharose CL-4B; DEAE-cellulose 29.0 3.10 17.9 [38]
C. testosteroni sp. Q-Sepharose; Superdex 200; Hydroxyapatite 60 68 79 [35]
R. rhodochrous NCIMB 11216 Ammonium sulfate precipitation; Anion-exchange FPLC; Sepharose Q; Suparose 12 HR 10/30 62 10.6 9.5 [43]
R. rhodochrous PA-34 Ammonium sulfate fractionation; 1st Sephacryl S-300 HR; 2nd Sephacryl S-300 HR; DEAE Toyopearl 650S 14.10 3.52 34.8 [39]
P. fluorescens DSM 7155 Phenyl-Sepharose FF; Mono Q; Superose 12 259 90 10 [44]
A. facilis 72W Ammonium sulfate precipitation; Sephadex G-25; Q-Sepharose 10.5 21 65 [48]
P. putida MTCC 5110 Ammonium sulfate fractionation; Superdex 200; Q-sepharose; Phenyl sepharose 35.01 3.26 10.15 [46]
A. niger K10 Ammonium sulfate precipitation; Sephacryl S-200; Q-Sepharose 18.7 91.6 24.3 [66]
P. abyssi GE5 Heat treatment; Q-Sepharose 23.85 141.9 88 [47]
F. solani O1 Phenyl sepharose; Sephacryl S-200; Q-Sepharose 9.9 156 25.9 [67]
F. solani IMI 196840 Phenyl sepharose; Sephacryl S-200; Q-Sepharose 20.3 144.0 26.9 [69]
  1. a Purification process only listed the adopted column chromatography techniques after cell-free extracts preparation.