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Table 1 The reported bacteria with nitrilase activity in previous literature

From: Nitrilases in nitrile biocatalysis: recent progress and forthcoming research

Bacteria

Formation type

Molecular weight (kDa)

Optimum pH / temperature (°C)

Stability pH / temperature (°C)

Substrate specificity

K m (M)

Reference

Pseudomonas sp.

Inducible

-

-

7.4-8.8 / -

N-Methyl/ethyl-3-cyano-4- methoxy-2-pyridone

1.9×10-5

[27]

Nocardia sp. NCIB 11216

Inducible

560

8 / -

-

Aromatic nitrile

-

[29]

Brevibacterium strain R312

Constitutive

-

7 / 35

- / below 30

-

2.5×10-2

[30]

Pseudomonas sp. 13

Inducible

1,000

9 / 55

7-11 / below 60

β-Cyano-L-alanine

8.3×10-2

[31]

Acinetobaeter sp. APN

Inducible

-

-

-

α-Aminonitriles

3.4×10-2

[32]

R. rhodochrous J1

Inducible

78

7.6 / 45

- / 20-50

Aliphatic, aromatic nitriles

-

[33, 34]

Comamonas testosteroni sp.

Inducible

-

-

-

Aliphatic nitriles

-

[35]

A. faecalis JM3

Inducible

275

7.5 / 45

7-8 / 20-50

Arylacetonitriles

3.3×10-4

[36]

Acinetobacter sp. AK226

Inducible

580

8 / 50

5.8-8 / below 60

Aliphatic, heterocyclic nitriles

1.2×10-2

[37]

A. faecalis ATCC 8750

Inducible

460

7.5 / 40-45

6.5-8 / below 50

Arylacetonitriles

5.8×10-3

[38]

R. rhodochrous PA-34

Inducible

45

7.5 / 35

- / below 35

Aromatic, aliphatic nitriles

4.5×10-2

[39]

R. rhodochrous K22

Inducible

650

5.5 / 50

- / below 55

Aliphatic nitriles

1.9×10-2

[40]

Klebsiella ozaenae

Constitutive

37

9.2 / 35

-

Bromoxynil

3.1×10-4

[41]

Bacillus pallidus Dac521

Inducible

600

7.6 / 65

6-9 / below 65

Aromatic nitriles

9.2×10-4

[42]

R. rhodochrous NCIMB 11216

Inducible

45.8

8 / 30

-

Aromatic nitriles

2.1×10-2

[43]

P. fluorescens DSM 7155

Inducible

130

9 / 55

-

Arylacetonitriles

8.7×10-5

[44]

N. globerula NHB-2

Inducible

-

-

-

Aromatic, unsaturated aliphatic nitriles

-

[45]

P. putida

Inducible

412

7 / 40

6.5-8 / below 50

Arylacetonitriles

1.3×10-2

[46]

Pyrococcus abyssi GE5

Inducible

60

7.4 / 80

4.5–8.5 / 60-90

Aliphatic dinitriles

9.5×10-3

[47]

Acidovorax facilis 72W

Inducible

570

- / 65

5-10 / below 60

Aliphatic dinitriles

5.6×10-2

[48]

Rhodococcus sp. NDB1165

Inducible

-

8 / 45

- / below 50

Aromatic and unsaturated aliphatic nitriles

0.1

[49]

Bradyrhizobium japonicum USDA110

Inducible

455

-

-

Mandelonitrile, phenylacetonitrile

2.6×10-4

[50]

P. aeruginosa 10145

Inducible

-

-

-

Aromatic nitriles

-

[51]

Halomonas nitrilicus sp. nov.

Inducible

-

-

-

Arylaliphatic nitriles

-

[52]

Bacillus subtilis ZJB-063

Constitutive

-

-

-

Arylacetonitriles

-

[53]

Alcaligenes sp. ECU0401

Constitutive

376

8 / 40

- / below 50

Aliphatic and aromatic nitriles

2.2×10-2

[54]

P. fluorescens Pf-5

-

138

7 / 45

- / below 65

Dinitriles

1.8×10-2

[55]

Streptomyces sp. MTCC 7546

Inducible

-

-

-

Aliphatic nitriles

-

[56]

Arthrobacter nitroguajacolicus ZJUTB06-99

Inducible

-

6.5 / 40

- / below 50

Aliphatic and aromatic nitriles

1.2×10-2

[57]

R. erythropolis ZJB-0910

Inducible

-

7.5 / 30

- / below 30

β-Hydroxy aliphatic nitrile

1.0×10-2

[58]

Geobacillus pallidus RAPc8

Inducible

600

-

-

Aromatic nitriles

-

[59]

A. faecalis ZJUTB10

Inducible

-

7.7–8.5 / 35

- / below 35

-

-

[60]

A. faecalis MTCC 10757

Inducible

-

8 / 35

-

Aliphatic and aromatic nitriles

-

[61]

  1. Note: “-” indicates no data available.