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Table 1 The reported bacteria with nitrilase activity in previous literature

From: Nitrilases in nitrile biocatalysis: recent progress and forthcoming research

Bacteria Formation type Molecular weight (kDa) Optimum pH / temperature (°C) Stability pH / temperature (°C) Substrate specificity K m (M) Reference
Pseudomonas sp. Inducible - - 7.4-8.8 / - N-Methyl/ethyl-3-cyano-4- methoxy-2-pyridone 1.9×10-5 [27]
Nocardia sp. NCIB 11216 Inducible 560 8 / - - Aromatic nitrile - [29]
Brevibacterium strain R312 Constitutive - 7 / 35 - / below 30 - 2.5×10-2 [30]
Pseudomonas sp. 13 Inducible 1,000 9 / 55 7-11 / below 60 β-Cyano-L-alanine 8.3×10-2 [31]
Acinetobaeter sp. APN Inducible - - - α-Aminonitriles 3.4×10-2 [32]
R. rhodochrous J1 Inducible 78 7.6 / 45 - / 20-50 Aliphatic, aromatic nitriles - [33, 34]
Comamonas testosteroni sp. Inducible - - - Aliphatic nitriles - [35]
A. faecalis JM3 Inducible 275 7.5 / 45 7-8 / 20-50 Arylacetonitriles 3.3×10-4 [36]
Acinetobacter sp. AK226 Inducible 580 8 / 50 5.8-8 / below 60 Aliphatic, heterocyclic nitriles 1.2×10-2 [37]
A. faecalis ATCC 8750 Inducible 460 7.5 / 40-45 6.5-8 / below 50 Arylacetonitriles 5.8×10-3 [38]
R. rhodochrous PA-34 Inducible 45 7.5 / 35 - / below 35 Aromatic, aliphatic nitriles 4.5×10-2 [39]
R. rhodochrous K22 Inducible 650 5.5 / 50 - / below 55 Aliphatic nitriles 1.9×10-2 [40]
Klebsiella ozaenae Constitutive 37 9.2 / 35 - Bromoxynil 3.1×10-4 [41]
Bacillus pallidus Dac521 Inducible 600 7.6 / 65 6-9 / below 65 Aromatic nitriles 9.2×10-4 [42]
R. rhodochrous NCIMB 11216 Inducible 45.8 8 / 30 - Aromatic nitriles 2.1×10-2 [43]
P. fluorescens DSM 7155 Inducible 130 9 / 55 - Arylacetonitriles 8.7×10-5 [44]
N. globerula NHB-2 Inducible - - - Aromatic, unsaturated aliphatic nitriles - [45]
P. putida Inducible 412 7 / 40 6.5-8 / below 50 Arylacetonitriles 1.3×10-2 [46]
Pyrococcus abyssi GE5 Inducible 60 7.4 / 80 4.5–8.5 / 60-90 Aliphatic dinitriles 9.5×10-3 [47]
Acidovorax facilis 72W Inducible 570 - / 65 5-10 / below 60 Aliphatic dinitriles 5.6×10-2 [48]
Rhodococcus sp. NDB1165 Inducible - 8 / 45 - / below 50 Aromatic and unsaturated aliphatic nitriles 0.1 [49]
Bradyrhizobium japonicum USDA110 Inducible 455 - - Mandelonitrile, phenylacetonitrile 2.6×10-4 [50]
P. aeruginosa 10145 Inducible - - - Aromatic nitriles - [51]
Halomonas nitrilicus sp. nov. Inducible - - - Arylaliphatic nitriles - [52]
Bacillus subtilis ZJB-063 Constitutive - - - Arylacetonitriles - [53]
Alcaligenes sp. ECU0401 Constitutive 376 8 / 40 - / below 50 Aliphatic and aromatic nitriles 2.2×10-2 [54]
P. fluorescens Pf-5 - 138 7 / 45 - / below 65 Dinitriles 1.8×10-2 [55]
Streptomyces sp. MTCC 7546 Inducible - - - Aliphatic nitriles - [56]
Arthrobacter nitroguajacolicus ZJUTB06-99 Inducible - 6.5 / 40 - / below 50 Aliphatic and aromatic nitriles 1.2×10-2 [57]
R. erythropolis ZJB-0910 Inducible - 7.5 / 30 - / below 30 β-Hydroxy aliphatic nitrile 1.0×10-2 [58]
Geobacillus pallidus RAPc8 Inducible 600 - - Aromatic nitriles - [59]
A. faecalis ZJUTB10 Inducible - 7.7–8.5 / 35 - / below 35 - - [60]
A. faecalis MTCC 10757 Inducible - 8 / 35 - Aliphatic and aromatic nitriles - [61]
  1. Note: “-” indicates no data available.