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Table 5 Primers used for cloning

From: “Direct cloning in Lactobacillus plantarum: Electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete”

Primer

Sequence 5’→3’

Tm [°C]

mrr_Lp_F

ATGAGTTATAAGCGTTGGAATG

60

mrr_Lp_R

TCAATCTTGTTCATAATAATATGC

60

hTff1_SacI_F

CGAGAGCTC GAATTCAGGTGTGATC

66

Tldh_amp_R

CTGCTGGTCGAC AAAAAGATTAAAAAAGCC

64

M13_R_NheI

CGACGAGCTAGC AGCCAGGAAACAGCTATGACC

64

Cat_F_NheI

CGACGAGCTAGC AATGTGGTCTTTATTCTTCAAC

58

M13_R_XhoI

CGACGACTCGAG AGCCAGGAAACAGCTATGACC

64

Cat_F_NheI

CGACGAGCTAGC AATGTGGTCTTTATTCTTCAAC

58

hTff1_NheI_F

CGACGAGCTAGC GAATTCAGGTGTGATC

66

Tldh_amp_XhoI_R

CTGCTGCTCGAG AAAAAGATTAAAAAAGCCGCTGC

62

CAT_seq_R

AGTCATTCTTTACAGGAGTCC

60

GG_hTFF1_sense

ATGAAGATCAAGTTAACTGTTTTAA

62

CAT_seq_back

GTTATTGGGATAAGTTAGAGC

58

p256miniori_for

CCCGCACGCATAGCGGTGC

66

  1. Restriction sites are underlined. Tm (melting temperature) was calculated with the program Gene Runner (Version 3.05, Hastings Software Inc., USA).