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Table 2 Transformation efficiency of LAB strains transformed with variously methylated plasmid pCD256

From: “Direct cloning in Lactobacillus plantarum: Electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete”

DNA source Relevant genotype Transfromation efficiency [cfu/μg DNA] of the following LAB strains
L. plantarum CD032 L. plantarum CD033 L. plantarum DSM20174 L. buchneri CD034 E. faecium CD036 L. lactis MG1363*
JM109 dam + dcm + 4.0 x 102 3.0 x 105 0 8.0 x 104 20 2.0 x 105
C2925 dam - dcm - 6.0 x 103 8.7 x 108 1.8 x 102 0 0 0
  1. Results are the arithmetic mean of four replicates. * transformed with pCDWV01.