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Table 2 Transformation efficiency of LAB strains transformed with variously methylated plasmid pCD256

From: “Direct cloning in Lactobacillus plantarum: Electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete”

DNA source

Relevant genotype

Transfromation efficiency [cfu/μg DNA] of the following LAB strains

L. plantarum CD032

L. plantarum CD033

L. plantarum DSM20174

L. buchneri CD034

E. faecium CD036

L. lactis MG1363*

JM109

dam + dcm +

4.0 x 102

3.0 x 105

0

8.0 x 104

20

2.0 x 105

C2925

dam - dcm -

6.0 x 103

8.7 x 108

1.8 x 102

0

0

0

  1. Results are the arithmetic mean of four replicates. * transformed with pCDWV01.