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Table 1 Transformation efficiency of L. plantarum CD033 with pCD256 isolated from different E. coli strains

From: “Direct cloning in Lactobacillus plantarum: Electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete”

DNA source

Relevant genotype

Transformation efficiency [cfu/μg DNA]

minimum

mean

maximum

JM109

dam+ dcm+

2.5 x 105

3.0 x 105

5.0 x 105

BL21 (DE3)

dam+ dcm-

3.0 x 105

4.1 x 105

4.7 x 105

GM33

dam- dcm+

1.6 x 105

2.3 x 105

3.0 x 105

C2925

dam- dcm-

5.0 x 108

8.7 x 108

9.7 x 108

  1. Transformation efficiencies are given as the minimum, the maximum and the arithmetic mean values of four replicates.
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Microbial Cell Factories

ISSN: 1475-2859

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