Skip to main content

Table 1 Transformation efficiency of L. plantarum CD033 with pCD256 isolated from different E. coli strains

From: “Direct cloning in Lactobacillus plantarum: Electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete”

DNA source Relevant genotype Transformation efficiency [cfu/μg DNA]
minimum mean maximum
JM109 dam+ dcm+ 2.5 x 105 3.0 x 105 5.0 x 105
BL21 (DE3) dam+ dcm- 3.0 x 105 4.1 x 105 4.7 x 105
GM33 dam- dcm+ 1.6 x 105 2.3 x 105 3.0 x 105
C2925 dam- dcm- 5.0 x 108 8.7 x 108 9.7 x 108
  1. Transformation efficiencies are given as the minimum, the maximum and the arithmetic mean values of four replicates.