Substrate cycles in Penicillium chrysogenum quantified by isotopic non-stationary flux analysis

Table 2 Substrate cycle fluxes at steady-state estimated from instationary ¹³ C metabolic flux analysis

Name	Reaction	flux [mmol/Cmol_BM/h]
r3.2	FBPase	7.25 ± 0.91
rz.2b	Trehalose	0.065 ± 0.014
rz.4a+ rz.4a2	Mannitol cycle	1.326 ± 0.162
rz.5a	Erythritol cycle	0.021 ± 0.017
rz.9	g6p phosphatase	10.6 ± 2.1
rz.9c	Polysaccharide degradation	6.47 ± 0.76
rz.8a	Pyr:m recycling	13.1 ± n.d.^*
rz.8d1	AcCoA recycling	11.3 ± n.d.^*

^*The flux standard deviation could not be determined (very high correlation) and was therefore taken out of the calculation. Further parameters/fluxes that were taken out during the statistical calculation are marked in Additional file 1: Table S5 with an asterix.

ISSN: 1475-2859